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Publication
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular
Matrix.
Authors Stephenson MK, Lenihan S, Covarrubias R, Huttinger RM, Gumina RJ, Sawyer DB,
Galindo CL
Submitted By Submitted Externally on 1/3/2018
Status Published
Journal Journal of visualized experiments : JoVE
Year 2016
Date Published 6/1/2016
Volume : Pages Not Specified : Not Specified
PubMed Reference 27340841
Abstract Fibrosis is a component of all forms of heart disease regardless of etiology,
and while much progress has been made in the field of cardiac matrix biology,
there are still major gaps related to how the matrix is formed, how
physiological and pathological remodeling differ, and most importantly how
matrix dynamics might be manipulated to promote healing and inhibit fibrosis.
There is currently no treatment option for controlling, preventing, or reversing
cardiac fibrosis. Part of the reason is likely the sheer complexity of cardiac
scar formation, such as occurs after myocardial infarction to immediately
replace dead or dying cardiomyocytes. The extracellular matrix itself
participates in remodeling by activating resident cells and also by helping to
guide infiltrating cells to the defunct lesion. The matrix is also a storage
locker of sorts for matricellular proteins that are crucial to normal matrix
turnover, as well as fibrotic signaling. The matrix has additionally been
demonstrated to play an electromechanical role in cardiac tissue. Most
techniques for assessing fibrosis are not qualitative in nature, but rather
provide quantitative results that are useful for comparing two groups but that
do not provide information related to the underlying matrix structure.
Highlighted here is a technique for visualizing cardiac matrix ultrastructure.
Scanning electron microscopy of decellularized heart tissue reveals striking
differences in structure that might otherwise be missed using traditional
quantitative research methods.




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