mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
Reduced cognitive function, increased blood-brain-barrier transport and
inflammatory responses, and altered brain metabolites in LDLr -/-and C57BL/6
mice fed a western diet.
Authors Rutkowsky JM, Lee LL, Puchowicz M, Golub MS, Befroy DE, Wilson DW, Anderson S,
Cline G, Bini J, Borkowski K, Knotts TA, Rutledge JC,
Submitted By Submitted Externally on 3/23/2018
Status Published
Journal PLoS ONE
Year 2018
Date Published
Volume : Pages 13 : e0191909
PubMed Reference 29444171
Abstract Recent work suggests that diet affects brain metabolism thereby impacting
cognitive function. Our objective was to determine if a western diet altered
brain metabolism, increased blood-brain barrier (BBB) transport and
inflammation, and induced cognitive impairment in C57BL/6 (WT) mice and
low-density lipoprotein receptor null (LDLr -/-) mice, a model of hyperlipidemia
and cognitive decline. We show that a western diet and LDLr -/- moderately
influence cognitive processes as assessed by Y-maze and radial arm water maze.
Also, western diet significantly increased BBB transport, as well as microvessel
factor VIII in LDLr -/- and microglia IBA1 staining in WT, both indicators of
activation and neuroinflammation. Interestingly, LDLr -/- mice had a significant
increase in 18F- fluorodeoxyglucose uptake irrespective of diet and brain
1H-magnetic resonance spectroscopy showed increased lactate and lipid moieties.
Metabolic assessments of whole mouse brain by GC/MS and LC/MS/MS showed that a
western diet altered brain TCA cycle and ß-oxidation intermediates, levels of
amino acids, and complex lipid levels and elevated proinflammatory lipid
mediators. Our study reveals that the western diet has multiple impacts on brain
metabolism, physiology, and altered cognitive function that likely manifest via
multiple cellular pathways.




Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.