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Publication
Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and
adipose core clock genes in mice and characterization of 24-h circulating CT-1
profiles in normal-weight and overweight/obese subjects.
Authors López-Yoldi M, Stanhope KL, Garaulet M, Chen XG, Marcos-Gómez B, Carrasco-Benso
MP, Santa Maria EM, Escoté X, Lee V, Nunez MV, Medici V, Martínez-Ansó E, Sáinz
N, Huerta AE, Laiglesia LM, Prieto J, Martínez JA, Bustos M, Havel PJ,
Moreno-Aliaga MJ
Submitted By Submitted Externally on 5/25/2018
Status Published
Journal FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Year 2017
Date Published 4/1/2017
Volume : Pages 31 : 1639 - 1649
PubMed Reference 28096235
Abstract Cardiotrophin (CT)-1 is a regulator of glucose and lipid homeostasis. In the
present study, we analyzed whether CT-1 also acts to peripherally regulate
metabolic rhythms and adipose tissue core clock genes in mice. Moreover, the
circadian pattern of plasma CT-1 levels was evaluated in normal-weight and
overweight subjects. The circadian rhythmicity of oxygen consumption rate (Vo2)
was disrupted in aged obese CT-1-deficient (CT-1-/-) mice (12 mo). Although
circadian rhythms of Vo2 were conserved in young lean CT-1-/- mice (2 mo), CT-1
deficiency caused a phase shift of the acrophase. Most of the clock genes
studied (Clock, Bmal1, and Per2) displayed a circadian rhythm in adipose tissue
of both wild-type (WT) and CT-1-/- mice. However, the pattern was altered in
CT-1-/- mice toward a lower percentage of the rhythm or lower amplitude,
especially for Bmal1 and Clock. Moreover, CT-1 mRNA levels in adipose tissue
showed significant circadian fluctuations in young WT mice. In humans, CT-1
plasma profile exhibited a 24-h circadian rhythm in normal-weight but not in
overweight subjects. The 24-h pattern of CT-1 was characterized by a pronounced
increase during the night (from 02:00 to 08:00). These observations suggest a
potential role for CT-1 in the regulation of metabolic circadian
rhythms.-López-Yoldi, M., Stanhope, K. L., Garaulet, M., Chen, X. G.,
Marcos-Gómez, B., Carrasco-Benso, M. P., Santa Maria, E. M., Escoté, X., Lee,
V., Nunez, M. V., Medici, V., Martínez-Ansó, E., Sáinz, N., Huerta, A. E.,
Laiglesia, L. M., Prieto, J., Martínez, J. A., Bustos, M., Havel, P. J.,
Moreno-Aliaga, M. J. Role of cardiotrophin-1 in the regulation of metabolic
circadian rhythms and adipose core clock genes in mice and characterization of
24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects.




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