mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
Trauma-Induced Nanohydroxyapatite Deposition in Skeletal Muscle is Sufficient to
Drive Heterotopic Ossification.
Authors Moore-Lotridge SN, Li Q, Gibson BHY, Martin JT, Hawley GD, Arnold TH, Saito M,
Tannouri S, Schwartz HS, Gumina RJ, Cates JMM, Uitto J, Schoenecker JG
Submitted By Submitted Externally on 12/12/2018
Status Published
Journal Calcified tissue international
Year 2018
Date Published 12/1/2018
Volume : Pages Not Specified : Not Specified
PubMed Reference 30515544
Abstract Heterotopic ossification (HO), or the pathologic formation of bone within soft
tissues, is a significant complication following severe injuries as it impairs
joint motion and function leading to loss of the ability to perform activities
of daily living and pain. While soft tissue injury is a prerequisite of
developing HO, the exact molecular pathology leading to trauma-induced HO
remains unknown. Through prior investigations aimed at identifying the causative
factors of HO, it has been suggested that additional predisposing factors that
favor ossification within the injured soft tissues environment are required.
Considering that chondrocytes and osteoblasts initiate physiologic bone
formation by depositing nanohydroxyapatite crystal into their extracellular
environment, we investigated the hypothesis that deposition of
nanohydroxyapatite within damaged skeletal muscle is likewise sufficient to
predispose skeletal muscle to HO. Using a murine model genetically predisposed
to nanohydroxyapatite deposition (ABCC6-deficient mice), we observed that
following a focal muscle injury, nanohydroxyapatite was robustly deposited in a
gene-dependent manner, yet resolved via macrophage-mediated regression over 28
days post injury. However, if macrophage-mediated regression was inhibited, we
observed persistent nanohydroxyapatite that was sufficient to drive the
formation of HO in 4/5 mice examined. Together, these results revealed a new
paradigm by suggesting the persistent nanohydroxyapatite, referred to clinically
as dystrophic calcification, and HO may be stages of a pathologic continuum, and
not discrete events. As such, if confirmed clinically, these findings support
the use of early therapeutic interventions aimed at preventing
nanohydroxyapatite as a strategy to evade HO formation.




Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.