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Administration of N-Acyl-Phosphatidylethanolamine Expressing Bacteria to Low
Density Lipoprotein Receptor-/- Mice Improves Indices of Cardiometabolic
Authors May-Zhang LS, Chen Z, Dosoky NS, Yancey PG, Boyd KL, Hasty AH, Linton MF, Davies
Submitted By Submitted Externally on 1/25/2019
Status Published
Journal Scientific reports
Year 2019
Date Published 1/1/2019
Volume : Pages 9 : 420
PubMed Reference 30674978
Abstract Obesity increases the risk for cardiometabolic diseases. N-acyl
phosphatidylethanolamines (NAPEs) are precursors of N-acylethanolamides, which
are endogenous lipid satiety factors. Incorporating engineered bacteria
expressing NAPEs into the gut microbiota retards development of diet induced
obesity in wild-type mice. Because NAPEs can also exert anti-inflammatory
effects, we hypothesized that administering NAPE-expressing bacteria to
low-density lipoprotein receptor (Ldlr)-/- mice fed a Western diet would improve
various indices of cardiometabolic disease manifested by these mice.
NAPE-expressing E. coli Nissle 1917 (pNAPE-EcN), control Nissle 1917 (pEcN), or
vehicle (veh) were given via drinking water to Ldlr-/- mice for 12 weeks.
Compared to pEcN or veh treatment, pNAPE-EcN significantly reduced body weight
and adiposity, hepatic triglycerides, fatty acid synthesis genes, and increased
expression of fatty acid oxidation genes. pNAPE-EcN also significantly reduced
markers for hepatic inflammation and early signs of fibrotic development. Serum
cholesterol was reduced with pNAPE-EcN, but atherosclerotic lesion size showed
only a non-significant trend for reduction. However, pNAPE-EcN treatment reduced
lesion necrosis by 69% indicating an effect on preventing macrophage
inflammatory death. Our results suggest that incorporation of NAPE expressing
bacteria into the gut microbiota can potentially serve as an adjuvant therapy to
retard development of cardiometabolic disease.


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