mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
Glutamate-Glutamine Transfer and Chronic Stress-Induced Sex Differences in
Cocaine Responses.
Authors Shimamoto A, Rappeneau V, Munjal H, Farris T, Davis C, Wilson A, Edwards M,
Moore C, Reynolds C, Meshul CK
Submitted By Submitted Externally on 1/25/2019
Status Published
Journal Neuroscience
Year 2018
Date Published 11/1/2018
Volume : Pages 391 : 104 - 119
PubMed Reference 30240589
Abstract Substance use disorders (SUD) often co-occur with other mental disorders such as
major depression (MD). Our previous findings revealed sex-dependent changes in
extracellular levels of glutamate (Glu) and glutamine (Gln) in the nucleus
accumbens (NAc) in Long-Evans rats that were exposed to 21?days of chronic
social defeat stress (CSDS), which models MD. The current study investigated the
role of a Gln transporter called sodium-coupled neutral amino acid transporter
subtype 1/2 (SNAT 1/2), phosphate-activated glutaminase (PAG), and astrocytic
glutamate transporter-1 (GLT-1) on CSDS animals exposed to cocaine. Before
cocaine exposure, CSDS males already showed decreased levels of SNAT 1/2 in the
NAc and prefrontal cortex (PFC) compared to non-CSDS controls. The reduction in
SNAT 1/2 levels was associated with an increase in Gln localization in the
mitochondrial outer membrane in accumbal glutamatergic nerve terminals
projecting from the PFC. CSDS females showed increased GLT-1 levels in the NAc
and PFC compared to non-CSDS controls. Both acute and repeated cocaine exposure
attenuated locomotor responses in CSDS males but increased those in CSDS
females. Cocaine reduced SNAT 1/2 levels in the NAc but increased them in the
PFC in CSDS males. Additionally, both PAG and GLT-1 levels were increased in the
PFC in CSDS males. On the other hand, cocaine reduced SNAT 1/2 and GLT-1 levels
in the NAc and PFC in CSDS females. Our results show that CSDS altered locomotor
responses upon cocaine exposure in a sex-dependent manner that may be mediated
by molecules associated with the Glu-Gln transfer.




Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.