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Anesthesia and Euthanasia of Brine Shrimp (Artemia franciscana).
Authors Darbyshire AK, Oliver KH, Dupont WD, Plummer WD, Jones CP, Boyd KL
Submitted By Submitted Externally on 2/22/2019
Status Published
Journal Journal of the American Association for Laboratory Animal Science : JAALAS
Year 2019
Date Published 1/1/2019
Volume : Pages 58 : 58 - 64
PubMed Reference 30497541
Abstract Invertebrates are often overlooked as laboratory animals, yet they are commonly
used in toxicology, developmental, cellular and molecular biology, and radiation
studies with euthanasia as an endpoint. Little is known regarding appropriate
euthanasia methods for invertebrate species, particularly for Artemia. Here, we
evaluated the AVMA-recommended 2-step method of euthanasia in brine shrimp
(Artemia franciscana). Artemia were exposed first to anesthetic solutions of 60%
alcohol, 2.5 mg/L eugenol, or 4 g/L tricaine methanesulfonate (TMS) and then
were transferred to euthanasia solutions of 70% alcohol, 95% alcohol, or 10%
neutral buffered formalin. We examined time to anesthesia, behavioral response
to anesthesia, anesthesia recovery, and time to euthanasia. Our results show
that 2.5 mg/L eugenol and 4 g/L TMS inconsistently achieved anesthesia. Although
60% alcohol produced anesthesia, the time to anesthesia varied among replicate
groups, and exposure resulted in an increase in abnormal behavior. We therefore
do not recommend any of the tested anesthetic solutions for use in Artemia.
Although all 3 euthanasia solutions were effective, more research is needed to
provide recommendations regarding euthanasia for this species.


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