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Publication
Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human
Pancreatic ß Cells for In Vitro and In Vivo Analysis.
Authors Saunders DC, Brissova M, Phillips N, Shrestha S, Walker JT, Aramandla R,
Poffenberger G, Flaherty DK, Weller KP, Pelletier J, Cooper T, Goff MT, Virostko
J, Shostak A, Dean ED, Greiner DL, Shultz LD, Prasad N, Levy SE, Carnahan RH,
Dai C, Sévigny J, Powers AC
Submitted By Submitted Externally on 3/13/2019
Status Published
Journal Cell Metabolism
Year 2019
Date Published 3/1/2019
Volume : Pages 29 : 745 - 754.e4
PubMed Reference 30449685
Abstract Identification of cell-surface markers specific to human pancreatic ß cells
would allow in vivo analysis and imaging. Here we introduce a biomarker,
ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase3), that is expressed
on the cell surface of essentially all adult human ß cells, including those from
individuals with type 1 or type 2 diabetes. NTPDase3 is expressed dynamically
during postnatal human pancreas development, appearing first in acinar cells at
birth, but several months later its expression declines in acinar cells while
concurrently emerging in islet ß cells. Given its specificity and membrane
localization, we utilized an NTPDase3 antibody for purification of live human ß
cells as confirmed by transcriptional profiling, and, in addition, for in vivo
imaging of transplanted human ß cells. Thus, NTPDase3 is a cell-surface
biomarker of adult human ß cells, and the antibody directed to this protein
should be a useful new reagent for ß cell sorting, in vivo imaging, and
targeting.





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