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Stimulation of water and calcium dynamics in astrocytes with pulsed infrared
Borrachero-Conejo AI, Adams WR, Saracino E, Mola MG, Wang M, Posati T, Formaggio
F, De Bellis M, Frigeri A, Caprini M, Hutchinson MR, Muccini M, Zamboni R,
Nicchia GP, Mahadevan-Jansen A, Benfenati V
Submitted Externally on 3/26/2020
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, REFERENCES
Volume : Pages
Astrocytes are non-neuronal cells that govern the homeostatic regulation of the
brain through ions and water transport, and Ca2+ -mediated signaling. As they
are tightly integrated into neural networks, label-free tools that can modulate
cell function are needed to evaluate the role of astrocytes in brain physiology
and dysfunction. Using live-cell fluorescence imaging, pharmacology,
electrophysiology, and genetic manipulation, we show that pulsed infrared light
can modulate astrocyte function through changes in intracellular Ca2+ and water
dynamics, providing unique mechanistic insight into the effect of pulsed
infrared laser light on astroglial cells. Water transport is activated and, IP3
R, TRPA1, TRPV4, and Aquaporin-4 are all involved in shaping the dynamics of
infrared pulse-evoked intracellular calcium signal. These results demonstrate
that astrocyte function can be modulated with infrared light. We expect that
targeted control over calcium dynamics and water transport will help to study
the crucial role of astrocytes in edema, ischemia, glioma progression, stroke,
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
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