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Publication
DSS-induced damage to basement membranes is repaired by matrix replacement and
crosslinking.
Authors Howard AM, LaFever KS, Fenix AM, Scurrah CR, Lau KS, Burnette DT, Bhave G,
Ferrell N, Page-McCaw A
Submitted By Submitted Externally on 4/6/2020
Status Published
Journal Journal of cell science
Year 2019
Date Published 4/1/2019
Volume : Pages 132 : Not Specified
PubMed Reference 30837285
Abstract Basement membranes are an ancient form of animal extracellular matrix. As
important structural and functional components of tissues, basement membranes
are subject to environmental damage and must be repaired while maintaining
functions. Little is known about how basement membranes get repaired. This
paucity stems from a lack of suitable in vivo models for analyzing such repair.
Here, we show that dextran sodium sulfate (DSS) directly damages the gut
basement membrane when fed to adult Drosophila DSS becomes incorporated into the
basement membrane, promoting its expansion while decreasing its stiffness, which
causes morphological changes to the underlying muscles. Remarkably, two days
after withdrawal of DSS, the basement membrane is repaired by all measures of
analysis. We used this new damage model to determine that repair requires
collagen crosslinking and replacement of damaged components. Genetic and
biochemical evidence indicates that crosslinking is required to stabilize the
newly incorporated repaired Collagen IV rather than to stabilize the damaged
Collagen IV. These results suggest that basement membranes are surprisingly
dynamic.




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