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Quantitative proteomics to study aging in rabbit liver.
Amin B, Ford KI, Robinson RAS
Submitted Externally on 4/13/2020
Mechanisms of ageing and development
Volume : Pages
187 : 111227
Aging globally effects cellular and organismal metabolism across a range of
mammalian species, including humans and rabbits. Rabbits (Oryctolagus cuniculus
are an attractive model system of aging due to their genetic similarity with
humans and their short lifespans. This model can be used to understand metabolic
changes in aging especially in major organs such as liver where we detected
pronounced variations in fat metabolism, mitochondrial dysfunction, and protein
degradation. Such changes in the liver are consistent across several mammalian
species however in rabbits the downstream effects of these changes have not yet
been explored. We have applied proteomics to study changes in the liver proteins
from young, middle, and old age rabbits using a multiplexing cPILOT strategy.
This resulted in the identification of 2,586 liver proteins, among which 45
proteins had significant p < 0.05) changes with aging. Seven proteins were
differentially-expressed at all ages and include fatty acid binding protein,
aldehyde dehydrogenase, enoyl-CoA hydratase, 3-hydroxyacyl CoA dehydrogenase,
apolipoprotein C3, peroxisomal sarcosine oxidase, adhesion G-protein coupled
receptor, and glutamate ionotropic receptor kinate. Insights to how alterations
in metabolism affect protein expression in liver have been gained and
demonstrate the utility of rabbit as a model of aging.
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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