twitter-logo @NationalMMPC
| Create Account | login
Regulation of gene expression during the fasting-feeding cycle of the liver
displays mouse strain specificity.
Authors Chi Y, Youn DY, Xiaoli AM, Liu L, Pessin JB, Yang F, Pessin JE
Submitted By Submitted Externally on 4/30/2020
Status Published
Journal The Journal of biological chemistry
Year 2020
Date Published 4/1/2020
Volume : Pages 295 : 4809 - 4821
PubMed Reference 32075912
Abstract The liver maintains metabolic homeostasis by integrating the regulation of
nutrient status with both hormonal and neural signals. Many studies on hepatic
signaling in response to nutrients have been conducted in mice. However, no
in-depth study is currently available that has investigated genome-wide changes
in gene expression during the normal physiological fasting-feeding cycle in
nutrient-sensitive and -insensitive mice. Using two strains of mice, C57BL/6J
and BALB/cJ, and deploying deep RNA-Seq complemented with quantitative RT-PCR,
we found that feeding causes substantial and transient changes in gene
expression in the livers of both mouse strains. The majority of significantly
changed transcripts fell within the areas of biological regulation and cellular
and metabolic processes. Among the metabolisms of three major types of
macronutrients (i.e. carbohydrates, proteins, and lipids), feeding affected
lipid metabolism the most. We also noted that the C57BL/6J and BALB/cJ mice
significantly differed in gene expression and in changes in gene expression in
response to feeding. In both fasted and fed states, both mouse strains shared
common expression patterns for about 10,200 genes, and an additional 400-600
genes were differentially regulated in one strain but not the other. Among the
shared genes, more lipogenic genes were induced upon feeding in BABL/cJ than in
C57BL/6J mice. In contrast, in the population of differentially enriched genes,
C57BL/6J mice expressed more genes involved in lipid metabolism than BALB/cJ
mice. In summary, these results reveal that the two mouse strains used here
exhibit several differences in feeding-induced hepatic responses in gene
expression, especially in lipogenic genes.


About MMPC
Animal Husbandry
Tests Data
Search Data
MMPC Centers


Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping

2017 National MMPC. All Rights Reserved.