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Hypoglycemia after Bariatric Surgery in Mice and Optimal Dosage and Efficacy of
Glucose Supplementation.
Authors Hsi ZY, Stewart LA, Lloyd KCK, Grimsrud KN
Submitted By Submitted Externally on 4/30/2020
Status Published
Journal Comparative medicine
Year 2020
Date Published 4/1/2020
Volume : Pages 70 : 111 - 118
PubMed Reference 32014086
Abstract The Roux-en-Y Gastric Bypass (RYGB) mouse model is a vital tool for studying the
pathophysiology of bariatric surgery and contributes greatly to research on
obesity and diabetes. However, complications including postsurgical hypoglycemia
can have profoundly negative effects. Unlike in humans, blood glucose (BG) is
not typically managed in postoperative rodents, despite their critical role as
translational models; without this management, rodents can experience
hypoglycemia, potentially impairing wound healing, decreasing survivability,
complicating interpretation of research data, and limiting translational
utility. In this project, we sought to identify an optimal method for minimally
invasive administration of dextrose in C57BL/6N (n = 16; 8 male, 8 female) mice.
To do so, we characterized BG pharmacokinetic profiles after subcutaneous and
oral-transmucosal (OTM) administration of dextrose. Compared with OTM dosage,
the subcutaneous route provided more consistent and reliable delivery of glucose
and did not cause significant adverse reactions. We then evaluated the frequency
of hypoglycemic events after RYGB in C57BL/6N mice (n = 16; 8 male, 8 female)
and the effects of subcutaneous dextrose supplementation on morbidity and
mortality. BG measurement and behavioral pain assessment (grimace test) were
performed for 3 d after surgery. Hypoglycemic (BG = 60 mg/dL) animals were
assigned to dose (5% dextrose SC) or no-dose treatment groups. Nearly all (87%)
mice became hypoglycemic; 2 of these mice died. No significant intergroup
difference in grimace score or mortality was detected. Overall, our results
demonstrate that hypoglycemia is a frequent adverse event after RYGB in mice and
that subcutaneous injection of dextrose is a safe and effective way to manage
hypoglycemia. Further studies are necessary to optimize the intervention
threshold and optimal dosage; regardless, we recommend glycemic management after
RYGB surgery in mice.


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