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Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids.
Vilgelm AE, Bergdorf K, Wolf M, Bharti V, Shattuck-Brandt R, Blevins A, Jones C,
Phifer C, Lee M, Lowe C, Hongo R, Boyd K, Netterville J, Rohde S, Idrees K,
Bauer JA, Westover D, Reinfeld B, Baregamian N, Richmond A, Rathmell WK, Lee E,
McDonald OG, Weiss VL
Submitted Externally on 9/28/2020
Volume : Pages
23 : 101408
Patient-derived cancer organoids hold great potential to accurately model and
predict therapeutic responses. Efficient organoid isolation methods that
minimize post-collection manipulation of tissues would improve adaptability,
accuracy, and applicability to both experimental and real-time clinical
settings. Here we present a simple and minimally invasive fine-needle aspiration
(FNA)-based organoid culture technique using a variety of tumor types including
gastrointestinal, thyroid, melanoma, and kidney. This method isolates organoids
directly from patients at the bedside or from resected tissues, requiring
minimal tissue processing while preserving the histologic growth patterns and
infiltrating immune cells. Finally, we illustrate diverse downstream
applications of this technique including in vitro high-throughput
chemotherapeutic screens, in situ immune cell characterization, and in vivo
patient-derived xenografts. Thus, routine clinical FNA-based collection
techniques represent an unappreciated substantial source of material that can be
exploited to generate tumor organoids from a variety of tumor types for both
discovery and clinical applications.
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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