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Sex differences in response to short-term high fat diet in mice.
Huang KP, Ronveaux CC, Knotts TA, Rutkowsky JR, Ramsey JJ, Raybould HE
Submitted Externally on 9/28/2020
Physiology & behavior
Volume : Pages
221 : 112894
Consumption of high-fat diet (HF) leads to hyperphagia and increased body weight
in male rodents. Female rodents are relatively resistant to hyperphagia and
weight gain in response to HF, in part via effects of estrogen that suppresses
food intake and increases energy expenditure. However, sex differences in energy
expenditure and activity levels with HF challenge have not been systemically
described. We hypothesized that, in response to short-term HF feeding, female
mice will have a higher energy expenditure and be more resistant to HF-induced
hyperphagia than male mice., Six-week-old male and female C57BL/6 J mice were
fed either low fat (LF, 10% fat) or moderate HF (45% fat) for 5 weeks, and
energy expenditure, activity and meal pattern measured using comprehensive
laboratory animal monitoring system (CLAMS)., After 5 weeks, HF-fed male mice
had a significant increase in body weight and fat mass, compared with LF-fed
male mice. HF-fed female had a significant increase in body weight compared with
LF-fed female mice, but there was no significant difference in fat mass. HF-fed
male mice had lower energy expenditure compared to HF-fed female mice, likely
due in part to reduced physical activity in the light phase. HF-fed male mice
also had increased energy intake in the dark phase compared to LF-fed male mice
and a reduced response to exogenous cholecystokinin-induced inhibition of food
intake. In contrast, there was no difference in energy intake between LF-fed and
HF-fed female mice., The data show that female mice are generally protected from
short-term HF-induced alterations in energy balance, possibly by maintaining
higher energy expenditure and an absence of hyperphagia. However, HF-feeding in
male mice induced weight and fat mass gain and hyperphagia. These findings
suggest that there is a sex difference in the response to short-term HF-feeding
in terms of both energy expenditure and control of food intake.
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
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