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Development of a novel murine model of lymphatic metastasis.
Banan B, Beckstead JA, Dunavant LE, Sohn Y, Adcock JM, Nomura S, Abumrad N,
Goldenring JR, Fingleton B
Submitted Externally on 9/28/2020
Clinical & experimental metastasis
Volume : Pages
37 : 247 - 255
Current laboratory models of lymphatic metastasis generally require either
genetically modified animals or are technically challenging. Herein, we have
developed a robust protocol for the induction of intralymphatic metastasis in
wild-type mice with reproducible outcomes. To determine an optimal injection
quantity and timeline for tumorigenesis, C57Bl/6 mice were injected directly
into the mesenteric lymph duct (MLD) with varying numbers of syngeneic murine
colon cancer cells (MC38) or gastric cancer cells (YTN16) expressing
GFP/luciferase and monitored over 2-4 weeks. Tumor growth was tracked via
whole-animal in vivo bioluminescence imaging (IVIS). Our data indicate that the
injection of tumor cells into the MLD is a viable model for lymphatic metastasis
as necropsies revealed large tumor burdens and metastasis in regional lymph
nodes. This protocol enables a closer study of the role of lymphatics in cancer
metastasis and opens a window for the development of novel approaches for
treatment of metastatic diseases.
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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