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Collagen 24 a1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose
Authors Xiong Weng, De Lin, Jeffrey T J Huang, Roland H Stimson, David H Wasserman, Li
Submitted By David Wasserman on 9/28/2020
Status Published
Journal International journal of molecular sciences
Year 2020
Date Published 8/10/2020
Volume : Pages 21 : 5738
PubMed Reference 32785142
Abstract Aberrant extracellular matrix (ECM) remodelling in muscle, liver and adipose
tissue is a key characteristic of obesity and insulin resistance. Despite its
emerging importance, the effective ECM targets remain largely undefined due to
limitations of current approaches. Here, we developed a novel ECM-specific mass
spectrometry-based proteomics technique to characterise the global view of the
ECM changes in the skeletal muscle and liver of mice after high fat (HF) diet
feeding. We identified distinct signatures of HF-induced protein changes between
skeletal muscle and liver where the ECM remodelling was more prominent in the
muscle than liver. In particular, most muscle collagen isoforms were increased
by HF diet feeding whereas the liver collagens were differentially but
moderately affected highlighting a different role of the ECM remodelling in
different tissues of obesity. Moreover, we identified a novel association
between collagen 24a1 and insulin resistance in the skeletal muscle. Using
quantitative gene expression analysis, we extended this association to the white
adipose tissue. Importantly, collagen 24a1 mRNA was increased in the visceral
adipose tissue, but not the subcutaneous adipose tissue of obese diabetic
subjects compared to lean controls, implying a potential pathogenic role of
collagen 24a1 in obesity and type 2 diabetes.


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