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Alterations in Gut Microbiota Do Not Play a Causal Role in Diet-independent
Weight Gain Caused by Ovariectomy.
Authors Sau L, Olmstead CM, Cui LJ, Chen A, Shah RS, Kelley ST, Thackray VG
Submitted By Submitted Externally on 2/22/2021
Status Published
Journal Journal of the Endocrine Society
Year 2021
Date Published 1/1/2021
Volume : Pages 5 : bvaa173
PubMed Reference 33324864
Abstract Acute estrogen deficiency in women can occur due to many conditions including
hyperprolactinemia, chemotherapy, GnRH agonist treatment, and removal of hormone
replacement therapy. Ovariectomized (OVX) rodent models, often combined with a
high-fat diet (HFD), have been used to investigate the effects of decreased
estrogen production on metabolism. Since evidence suggests that gut microbes may
facilitate the protective effect of estrogen on metabolic dysregulation in an
OVX + HFD model, we investigated whether the gut microbiome plays a role in the
diet-independent weight gain that occurs after OVX in adult female mice. 16S
rRNA gene sequence analysis demonstrated that OVX was not associated with
changes in overall gut bacterial biodiversity but was correlated with a shift in
beta diversity. Using differential abundance analysis, we observed a difference
in the relative abundance of a few bacterial taxa, such as Turicibacter, 3 to 5
weeks after OVX, which was subsequent to the weight gain that occurred 2 weeks
postsurgery. A cohousing study was performed to determine whether exposure to a
healthy gut microbiome was protective against the development of the metabolic
phenotype associated with OVX. Unlike mouse models of obesity, HFD
maternal-induced metabolic dysregulation, or polycystic ovary syndrome,
cohousing OVX mice with healthy mice did not improve the metabolic phenotype of
OVX mice. Altogether, these results indicate that changes in the gut microbiome
are unlikely to play a causal role in diet-independent, OVX-induced weight gain
(since they occurred after the weight gain) and cohousing with healthy mice did
not have a protective effect.


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