mmpc-logo
twitter-logo @NationalMMPC
| Create Account | login
Strain
B6(Cg)-Snord116tm1.1Uta

Summary Data Summary
Official Name B6(Cg)-Snord116tm1.1Uta
Common Name Snord116del
Description Snord116 cluster knockout: Two individual targeting vectors
were used to place a loxP site (and an Frt1-flanked PGK-neo
cassette) upstream, and a loxP site (and an Frt5-flanked
puromycin resistance/TK cassette) downstream of the Snord116
cluster. The upstream targeting vector was transfected into
C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and
correctly targeted ES cells were next transfected with the
downstream targeting vector. Doubly targeted ES cells were
then transiently transfected with a cre expressing plasmid.
The resulting 1-loxP ES cells (with the Snord116 cluster
deleted and a single loxP site remaining) were injected into
recipient blastocysts.
Development Status Not specified/Other
Creation Method knockout
Phenotype Description mouse model of Prader-Willi syndrome
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1
Publications 1


Investigators
NameInstitution
Dag YasuiUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Snord116knockoutknockoutNot Specified


Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.