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Catalog Item
Plasma lipids
Common Name(s): Lipid Analysis

CenterVanderbilt University School of Medicine
Center CoreAnalytical Resources Core
Test No.V3070
Test PriceInquire for Pricing. Analytical services are provided as part of in vivo projects.
DescriptionEnzymatic Free Fatty Acid Test Price - $15.60

Total plasma cholesterol and triglyceride are measured by standard enzymatic
assays. HDL cholesterol is measured with the enzymatic method after
precipitation of VLDL and LDL using dextran sulfate and Mg++. Using these data
LDL cholesterol can be calculated using the Friedewald equation, if triglyceride
levels are below 400 mg/dl. Investigators may request a total plasma lipid
profile or specific plasma lipid measurements.

Free fatty acids are extracted from plasma using heptane/isopropanol. The
heptane layer containing FFA is removed, plated on silica gel plates and
developed in petroleum ether, ethyl ether, and acetic acid. The FFA band is
scraped from the plate and FFA are eluted with heptane /isopropanol. The solvent
is removed, and the FFAs are methylated. Methylated fatty acids are analyzed by
gas chromatography. Depending on the assay a variety of chromatograph conditions
and columns are utilized. A computer identifies each fatty acid peak and can
provide data in a number of different ways including quantitation of mass of
fatty acid, percent distribution of fatty acids present, quantitation of total
lipid in the sample.
DataData will be collected for this catalog item
Keywordscholesterol, fatty acids, lipids, metabolism, obesity
Tissuesadipose tissue



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