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Catalog Item
Short chain fatty acid analysis by gas liquid chromatography
Common Name(s): Lipid Analysis

CenterVanderbilt University School of Medicine
Center CoreAnalytical Resources Core
Test No.V3074
Test PriceInquire for Pricing. Analytical services are provided as part of in vivo projects.
DescriptionPlasma short chain fatty acids are analyzed by the following procedure: to 200
ul of EDTA plasma in a 1.5 ml Eppendorf microfuge tube is added 20 µl of
internal standard and 1 ml of absolute ethanol. The sample is mixed thoroughly,
centrifuged, and the supernatant is recovered. The sample is evaporated using a
Speed Vac and dissolved in 15 µl water, and prior to injection 5 µl of
orthophosphoric acid (25%) is added. The short chain fatty acids are separated
on a 6' x 2 mm glass column packed with SP-1200/1%H3PO4 on 80/100 Chromosorb W
AW.
DataData will be collected for this catalog item
KeywordsGCMS, lipids, metabolism, short chain fatty acid
Tissues



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