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Catalog Item
Fast Protein Liquid Chromatographic (FPLC) Lipoproteins Separations
Common Name(s): Lipid Analysis, Lipoprotein composition

CenterVanderbilt University School of Medicine
Center CoreAnalytical Resources Core
Test No.V3075
Test PriceInquire for Pricing. Analytical services are provided as part of in vivo projects.
DescriptionLipoprotein fractions are isolated using columns arranged in tandem to achieve
complete resolution of the major lipoprotein classes from 1-2 ml of plasma. The
columns are equilibrated in 50 mM phosphate-buffered saline and calibrated using
lipoprotein fractions isolated by ultracentrifugation. Fractions (0.5 ml) are
collected and the appropriate tubes containing the desired lipoprotein
fraction(s) combined. The position of the major lipoprotein classes are
determined by cholesterol (or triglyceride) assay on the column fractions using
a microtiter plate enzyme-based assay. As an alternative method lipoproteins can
be isolated by fast protein liquid chromatography.

This includes analysis of the composition of the fraction (protein and lipid) as
well as morphologic analysis (sizing) by negative stain electron microscopy. For
compositional analysis the lipoprotein fractions protein is analyzed using the
BCA method with a modification to eliminate lipid interference. The samples are
then lyophilized and delipidated using ethanol and ether. Lipid components are
separated by TLC and analyzed by GLC and/or colorimetric assays.
DataData will be collected for this catalog item
Keywordscholesterol, fatty acids, lipids, lipoproteins, metabolism
Tissuesadipose tissue



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