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Catalog Item
Hyperglycemic clamp
Common Name(s): Hyperglycemic clamp

CenterYale University School of Medicine
Center CoreMetabolic Phenotyping Core
Test No.Y4003
Test Price$430.00
DescriptionThe procedure involves chronic cannulation of the jugular vein which is
necessary for The surgery is performed at 4-5 days prior to the hyperglycemic
clamp to establish a chronic catheter for intravenous infusion of substances
(e.g., glucose, insulin) during the clamp. For this, a mouse is anesthetized
with an intraperitoneal injection of ketamine and xylazine, and a catheter is
inserted in the right jugular vein. On the day of clamp experiment, an
overnight-fasted mouse is placed in an over-sized restrainer (i.e., rat-sized)
for the experiment to be conducted in awake and minimally-stressed state. The
tail is tethered using a tape for 2 hours prior to the start of experiment for
acclimatization. A 3-way connector is attached to the jugular vein catheter for
intravenous infusion, and the blood samples are obtained from the tail vessels
requiring a small tail cut. A 2-hour hyperglycemic clamp is conducted with a
variable infusion of 20% glucose to raise and maintain plasma glucose
concentrations at ~16 mM. Blood samples (20 ml) are collected at 10-20 min
intervals for the immediate measurement of plasma glucose concentrations using
Beckman Glucose Analyzer. The area under curve of plasma glucose and insulin
profiles is assessed to determine glucose-induced insulin secretion in vivo
(i.e., pancreatic ß-cell function).
DataNo data will be collected for this catalog item
Keywordsclamp, diabetes, glucose, insulin, insulin action, insulin resistance, insulin
secretion
Tissuespancreas



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Phenotype Assays


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