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Experiment

Circulating lipid levels in Atf3 KO mice fed high fat diet
Summary Data Summary
Investigator Villablanca, Amparo
Description Mice (8 weeks of age) are fed either a low-fat diet (Harlan cat. no.TD.08485) or
high-fat diet (Harlan cat. no. TD.88137) for 8 weeks. They have ad lib access to
food and water. Animals at the age of 16 weeks are fasted roughly 6-13 hours
prior to euthanization by cardiac puncture and exsanguination. Blood is drawn by
cardiac puncture and collected in serum separator tubes. Samples are spun and
serum fraction is immediately drawn off and frozen at -80C until use.

Applicable research area(s): Neurobiology, Cardiovascular
Status Completed
Public Release 2/2/2019
Animal Age Measured In: week(s) post-natal (w)
Data Analysis
TypeCount
Animals24
Experimental Conditions5
Catalog Items4
Curation Info (# flags)0
Phenotype Assays6
Phenotype Measurements144
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
6
6
0
Atf3 (-/-)
6
6
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Teklad - 08485
Harlan Teklad TD.08485 Low Fat Control Diet Purified Diet, Modification of TD.88137, Low Fat & No Added Cholesterol, Reduced Sucrose.
 Teklad - 88137
Harlan Teklad TD.88137 (Western Diet)

Experimental Factor: Tissue Source

 serum
serum



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