MMPC :: Experiment

Experiment

Circulating lipid levels of B6 mice treated with niclosamide, cholestyramine,
and metformin

Summary

Data Summary

Investigator	Shengkan, Jin
Description	We had the C57BL6/J mice randomized into 5 groups. These mice were all fed on high-fat diet. Group 1 is controls without any treatment. Group 2 is niclosamide treatment group with oral treatment of niclosamide mixed into diet 2000ppm. Group 3 in metformin treatment group with oral treatment of metformin in water to achieve dosage of 75 mg/kg/d. Group 4 is synergistic treatment group with both niclosamide treatment group with oral treatment of niclosamide mixed into diet 2000ppm plus 20000ppm cholestyramine mixed into diet. Group 5 is synergistic treatment group with both niclosamide treatment group with oral treatment of niclosamide mixed into diet 2000ppm plus 20000ppm cholestyramine mixed into diet and metformin treatment. Blood samples were collected at age of 36 weeks after 5 hour fast. Each sample contains ~50 micro liters of plasma. Applicable research area(s): Diabetes, Metabolism, Endocrine deficiency
Status	PhenStat Analysis Verified
Public Release	3/21/2019
Animal Age	Measured In: week(s) post-natal (w)
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Data Analysis

DOWNLOAD ALL DATA (.csv)

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	0	45	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	Metformin	Metformin is a drug approved by the U.S. Food and Drug Administration as a prescription medication to treat diabetes. This medication is used to decrease hepatic (liver) glucose production, to decrease GI glucose absorption and to increase target...
	Vehicle	Vehicle - Control
	Experimental Factor: Drug Dose Units
	mg/kg/d	milligrams per kilograms per day
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Experimental Factor: Mouse Diet
	Research Diets - D09032705G	Rodent diet 40% kcal fat, 43% kcal carbohydrate, 17% kcal protein
	Research Diets - D16012604	Rodent diet 40% kcal fat, 43% kcal carbohydrate, 17% kcal protein supplemented with 2000 ppm niclosamide ethanolamine (NEN)
	Research Diets - D16012605	Rodent diet 40% kcal fat, 43% kcal carbohydrate, 17% kcal protein supplemented with 2000 ppm niclosamide ethanolamine (NEN) and 2% cholestyramine (COL)
	Experimental Factor: Tissue Source
	plasma (EDTA)	plasma (EDTA)

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
cholesterol, total	TC
triglycerides	TG
non-esterified free fatty acids	NEFA
cholesterol, HDL, plasma	HDL-cholesterol
cholesterol, LDL and VLDL	LDL/VLDL

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