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Experiment

Circulating FFA levels in B6 mice treated with ethanol and anti-Agrp RNAi
Summary Data Summary
Investigator Xu, Allison
Description Wildtype C57BL/6J mice were treated with RNAi reagents against AgRP or a control
RNAi (NC). Mice were given oral gavage of alcohol. Samples provided are plasma
from these mice, with the goal to determine if NEFA levels are different among
different genotypes and treatments. Ethanol gavage solution (5 g ethanol per kg
of body weight) Prepare a 31.5% (vol/vol) ethanol solution (0.25 g ml - 1
ethanol) by mixing 6.6 ml of 95% ethanol with 13.4 ml of water.

RNAi reagents:
Agrp-DsiRNAi (Integrated DNA Technologies Inc, MMC.RNAI.N007427.12.1. 5 µg per
mouse)
Negative control (Integrated DNA Technologies Inc, Ds NC1, 5 µg per mouse).
Mice got two treatments during a 22-hour period. Mice were housed at 30 degree
thermoneutral conditions."
Status PhenStat Analysis Verified
Public Release 10/29/2020
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals22
Experimental Conditions9
Catalog Items1
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements44
Histology Images0
Publications0
Phenotypes1


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
22
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Ethanol
Ethanol
 RNAi-Agrp
RNA interference targeting the Agrp gene
 Vehicle
Vehicle - Control

Experimental Factor: Drug Delivery Route

 IP
The drug was delivered intraperitoneal.
 PO
The drug was delivered orally

Experimental Factor: Drug Dose Units

 µg twice
micrograms two doses
 g/kg
grams per kilogram

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 LabDiet 5058
PicoLab Mouse Diet 20, 20.0% protein (Calories: 23% protein, 22% Fat, 55% Carbohydrate)

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)
Experimental Factor Values


Phenotype Assays Add / Edit


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