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Experiment

Circulating lipid, glucose, insulin and HbA1c levels in AC5 KO mice fed high fat
diet
Summary Data Summary
Investigator Navedo, Manuel
Description To determine the effects of hyperglycemia on vascular reactivity. Mice were
euthanize by sodium pentobarbita (IP injection) and blood samples collected at
endpoint by punturing the heart. Whole blood samples were collected in EDTA
tubes. For serum samples were allowed to clot (15 mins, room T) spined down, and
the supernatant transfered toa clean tube. Samples were stores at -20 ?. WT and
Adenilyl cyclase mice were fed a high fat or a low fat control for 12 weeks (see
table). Wt and AC5 KO Mice had adlibitum acces to food and water.
Status Completed
Public Release 1/10/2021
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal CountNo data
Data Analysis
TypeCount
Animals24
Experimental Conditions5
Catalog Items5
Curation Info (# flags)3
Phenotype Assays7
Phenotype Measurements168
Histology Images0
Publications0
Phenotypes1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
9
0
Adcy5tm1Yish, AC5 KO
0
15
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 Research Diets - D12450J
Rodent Diet with 10% fat, 70% carbohydrate, 20% protein
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)



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