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Experiment

Circulating lipid levels and tumor interstitial fluid lipid levels in B6 mice
implanted with melanoma
Summary Data Summary
Investigator Kaech, Susan
Description to examine LDL and HDL levels in tissue interstitial fluid. C57 B6 mice were
implanted with meolanoma (B16 or YUMMER). 3 weeks later, mice were sac'ed and
tumor interstitial fluid was collected by centrifugation.Paired serum was also
obtained by retro-orbital bleeding. The hypothesis is that there is differential
enrichment of LDL or HDL in tumor interstitial fluid compared to serum.
Status Completed
Public Release 3/22/2021
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals10
Experimental Conditions5
Catalog Items2
Curation Info (# flags)2
Phenotype Assays6
Phenotype Measurements60
Histology Images0
Publications0
Phenotypes1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
10
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 LabDiet 5053
PicoLab Rodent Diet 20, 21.0% protein (Calories: 24.5% protein, 13.1% Fat, 62.4% Carbohydrate)

Experimental Factor: Tissue Source

 Interstitial fluid -tumor
Interstitial fluid -tumor
 serum
serum



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