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Experiment

Circulating lipid levels in Ldlr KO mice fed high fat diet
Summary Data Summary
Investigator Altman, Robin
Description Mice (5 weeks of age) are fed either a chow diet or high-fat diet (Harlan cat.
no. TD.88137) for 8 weeks. They have ad lib access to food and water. Animals
are fasted roughly 12-18 hours prior to euthanization by cardiac puncture and
exsanguination. Blood is drawn by cardiac puncture and collected in serum
separator tubes. Samples are spun and serum fraction is immediately drawn off
and frozen at -80C until use.

Applicable research area(s): Neurobiology, Cardiovascular
Status Completed
Public Release 5/15/2017
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagAnimal Count
Data Analysis
TypeCount
Animals18
Experimental Conditions5
Catalog Items4
Curation Info (# flags)1
Phenotype Assays4
Phenotype Measurements72
Histology Images0
Publications0
Phenotypes3


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
6
0
0
C57BL/6J-Ldlr-/-
6
6
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...
 Teklad - 88137
Harlan Teklad TD.88137 (Western Diet)

Experimental Factor: Tissue Source

 serum
serum



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