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Strain
B6;129S4-Apoa4tm1Bres/J

Summary Data Summary
Official Name B6;129S4-Apoa4tm1Bres/J
Common Name B6;129S4-Apoa4tm1Bres/J
Description Description
Mice homozygous for this targeted mutation are viable and
fertile with no protein detectable in plasma, liver, or
intestinal tissues. Homozygotes have decreased plasma levels
of cholesterol, free fatty acids, and high density (HDL) and
very low density lipoprotein (VLDL). These mice may be
useful in studies of lipid metabolism, atherosclerosis,
heart disease, appetite regulation, intestinal lipid
absorption, or inflammatory bowel disease.

Development
A targeting vector was designed to replace exon 1 of the
endogenous gene with a neomycin resistance gene. This
construct was electroporated into 129S4/SvJae-derived J1
embryonic stem (ES) cells. Correctly targeted ES cells were
injected into C57BL/6J blastocysts. Chimeric mice were bred
with C57BL/6J and the resulting heterozygous offspring were
bred together for many generations prior to arrival at The
Jackson Laboratory.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6J
Breeding Type backcross
Breeding Generations 5
Phenotype Description Impaired weight gain on a high fat diet and reduced
adiposity
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1
Publications 1


Investigators
NameInstitution
Richard B WeinbergWake Forest University Baptist Medical Center


Genomic Information
GeneAllele 1Allele 2Protocol
Apoa4knockoutknockoutNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Impact of murine intestinal apolipoprotein A-IV expression on regional lipid absorption, gene expression, and growth.
Simon T, Cook VR, Rao A, Weinberg RB
Journal of lipid research, 2011 (52), 1984 - 94
Submitted Externally
21840868
Published

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