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Strain
C57BL/6-Snord116tm1.1Uta/+

Summary Data Summary
Official Name C57BL/6-Snord116tm1.1Uta/+
Common Name paternal Snord116del -/+
Description Heterozygous deletion male mice were bred with C57BL/6 WT
females to generate paternal deletion Snord116del+/- and WT
littermates.

Snord116 del: Two individual targeting vectors were used to
place a loxP site (and an Frt1-flanked PGK-neo cassette)
upstream, and a loxP site (and an Frt5-flanked puromycin
resistance/TK cassette) downstream of the Snord116 cluster.
The upstream targeting vector was transfected into
C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and
correctly targeted ES cells were next transfected with the
downstream targeting vector. Doubly targeted ES cells were
then transiently transfected with a cre expressing plasmid.
The resulting 1-loxP ES cells (with the Snord116 cluster
deleted and a single loxP site remaining) were injected into
recipient blastocysts.
Development Status Not specified/Other
Creation Method knockout
Maternal Strain C57BL/6
Paternal Strain B6(Cg)-Snord116tm1.1Uta/J
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 2
Publications 1


Investigators
NameInstitution
Dag YasuiUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Snord116knockoutwildNot Specified


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