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Strain
B6.129S6-Gpr17tm1a(KOMP)Mbp Agrptm1(cre)Lowl

Summary Data Summary
Official Name B6.129S6-Gpr17tm1a(KOMP)Mbp Agrptm1(cre)Lowl
Common Name C57BL/6-Gpr17tm1a(KOMP)Mbp Agrptm1(cre)Lowl
Description An IRES-cre-frt-kan/neo-frt cassette was inserted in frame
in exon 3 after the stop codon via homologous recombination.
The kan/neo cassette was subsequently removed via in vivo
Flp mediated recombination. Crosses to reporter mice
detected Cre activity in AGRP+ neurons.

The L1L2_Bact_P cassette was inserted at position 31948465
of Chromosome 18 upstream of the critical exon(s) (Build
GRCm38). The cassette is composed of an FRT site followed by
lacZ sequence and a loxP site. This first loxP site is
followed by neomycin under the control of the human
beta-actin promoter, SV40 polyA, a second FRT site and a
second loxP site. A third loxP site is inserted downstream
of the targeted exon(s) at position 31946900. The critical
exon(s) is/are thus flanked by loxP sites. A "conditional
ready" (floxed) allele can be created by flp recombinase
expression in mice carrying this allele. Subsequent cre
expression results in a knockout mouse. If cre expression
occurs without flp expression, a reporter knockout mouse
will be created.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 2
Experiments 1
Publications 1


Investigators
NameInstitution
Domenico AcciliColumbia University


Genomic Information
GeneAllele 1Allele 2Protocol
Gpr17knockin (lox)knockin (lox)Not Specified
Agrpknockin (Cre)knockin (Cre)Not Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Gpr17 in AgRP Neurons Regulates Feeding and Sensitivity to Insulin and Leptin.
Ren H, Cook JR, Kon N, Accili D
Diabetes, 2015 (64), 3670 - 9
Submitted Externally
26180086
Published

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