Isotope Tracers in Metabolic Research
Announcing an NIH/MMPC sponsored course:
11th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis

October 29 - November 2, 2018
Homewood Suites by Hilton® Nashville Vanderbilt
2400 West End Avenue
Nashville, Tennessee 37203 (also where the course takes place)
615-340-8000

Course Co-Directors: Owen P. McGuinness, PhD, Elizabeth J. Parks, PhD

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

Welcome to the Eleventh annual course which provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in humans and in animals. The course is designed for beginners as well as for those with experience who wish to expand their capabilities to more sophisticated problems. The faculty has expertise in a wide variety of applications and methodologies. Techniques are taught for the investigation of whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation in humans, animals and cells. Basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR are discussed, including examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Attendees can discuss their research projects in one-on-one mentoring sessions with faculty

Monday, October 29, 2018

Basic tracer theory
Basic characteristics of radioactive isotopes
General principles of mass spectrometry
Specific activity and isotopic enrichment (GC-MS)
Methods of mass spectrometry analysis MS, LC-MS
Problem solving sessions

Tuesday, October 30, 2018

Tracer kinetics (single pool models)
Oxidation and synthesis rates
Glucose metabolism (clamp studies)
Lipid metabolism (basic kinetics)
Introduction to the NIH grant process
Practical application of the insulin clamp (breakout session)
  • Human
  • Animals

Wednesday, October 31, 2018

Using positional isotopomer analysis to assess pathway fluxes using NMR
Protein metabolism

Thursday, November 1, 2018

Energy expenditure with doubly labeled water
Synthesis rates with deuterated water: proteins, fatty acids sterols, glucose, nucleic acids
Mass isotopomer distribution analysis: polymer synthesis, multiple flux pathways, TCA cycle, anaplerosis
Metabolic flux analysis workshop using MFA software suite

Friday, November 2, 2018

Small Group sessions
  • Working with Mass Spec data
  • Lipid flux
  • Carbohydrate flux
  • Protein flux
Pathway discovery via association of isotopomer analysis and metabolomics.
Discussion of inherently difficult problems

FOR COURSE DETAILS OR SUGGESTIONS: Eann Malabanan (isotope.tracer@vanderbilt.edu) (615-343-1065)

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Director & Faculty Information
Faculty:
Julio AYALA, PhDCleveland, OH
Henri BRUNENGRABER, MD PhDCleveland, OH
Gary CLINE, PhDNew Haven, CT
Stephanie CHUNG, MBBSBethesda, MD
Melanie CREE GREEN, MD PhDDenver, CO
Joanne KELLEHER, PhDBoston, MA
Maren LAUGHLIN, PhDBethesda, MD
Owen P. McGUINNESS, PhDNashville, TN -- Course Co-Director
Matthew MERRITT, PhDDallas, TX
Elizabeth J. PARKS, PhDColumbia, MO -- Course Co-Director
Stephen PREVIS, PhDRahway, NJ
Michelle PUCHOWICZ, PhDMemphis, TN
David H. WASSERMAN, PhDNashville, TN
Robert R. WOLFE, PhDLittle Rock, AR
Jamey YOUNG, PhDNashville, TN

Sponsored by:
This course is supported by The National Institute of Diabetes and Digestive and Kidney Diseases and Mouse Metabolic Phenotyping Centers.

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Course Information
2018 Course Syllabus:
Click here to download course syllabus.

COURSE REGISTRATION & MATERIAL
  • Registration opens on June 1, 2018
  • Registration is available online (Register click here) Registration is limited to 90 participants
  • Course registration deadline: Monday, October 1, 2018
  • Please refer to ‘Registration link’ for list of fees (credit cards accepted)
  • Registration fee includes:
    ~ breakfast, lunch and snacks
  • Please bring a Wi-Fi enable device to download and view all course material.
  • NOTE: The following book is also very useful "Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis" by Robert R. Wolfe and David L. Chinkes, 2nd Edition (2005 Wiley-Liss).
  • We have a small number of scholarships available for postdoctoral fellows or students to cover the registration fee. If you want to be considered for a scholarship, please register. Then send no more than a one page explanation of how the course would benefit your training in metabolic research as well as a letter from your mentor indicating your eligibility to isotope.tracer@vanderbilt.edu by 08.13.2018 (You will be notified by 8.17.18). Please do not pay for the registration until you hear from us as to whether you have been awarded the scholarship. We have limited ability to refund credit card transactions.

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ACCOMMODATIONS & INFORMATION
To book a room at the discount rate, please call 615-340-8000 or go directly to the following link:
http://homewoodsuites.hilton.com/en/hw/groups/personalized/B/BNAVBHW-T18-20181028/index.jhtml?WT.mc_id=POG
and mention the identifier:
group code “T18” or group name “Vanderbilt Tracer Course 2018”

Reservations must be made on or before 9/29/18

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