Isotope Tracers in Metabolic Research
Announcing an NIH/MMPC sponsored course:
10th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis

October 30 - November 3, 2017
Homewood Suites by Hilton® Nashville Vanderbilt
2400 West End Avenue
Nashville, Tennessee 37203
(also where the course takes place)

Course Co-Directors: Owen McGuinness, PhD, Henri Brunengraber, MD, PhD and Robert Wolfe, PhD

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

Welcome to the tenth annual course which provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in humans and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies. Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered. Course material will be available for download from

Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.

Monday, October 30, 2017

Basic characteristics of radioactive, stable isotope tracers.
General principles of mass spectrometry.
Isotopic enrichment using GC-MS.
Methods of mass spectrometry analysis.
Measurement of specific activity.

Tuesday, October 31, 2017

Tracer kinetics (single pool models).
Oxidation and synthesis rates.
Glucose metabolism (clamp studies).
Lipid metabolism (basic kinetics).

Wednesday, November 1, 2017

Pathway fluxes using NMR isotopomer analysis.
Methods in protein metabolism.

Thursday, November 2, 2017

Energy expenditure with doubly labeled water.
Synthesis rates with deuterated water: proteins, fatty acids sterols, glucose, nucleic acids.
Mass isotopomer distribution analysis: polymer synthesis, multiple flux pathways, TCA cycle, anaplerosis.

Friday, November 3, 2017

Pathway discovery via association of isotopomer analysis and metabolomics.
Inherently difficult problems.

FOR COURSE DETAILS OR SUGGESTIONS: Eann Malabanan ( (615-343-1065)

Director & Faculty Information
Henri BRUNENGRABER, MD PhDCleveland, OH -- Course Co-Director
Gary CLINE, PhDNew Haven, CT
Melanie CREE GREEN, MD PhDDenver, CO
Joanne KELLEHER, PhDBoston, MA
Maren LAUGHLIN, PhDBethesda, MD
Owen McGUINNESS, PhDNashville, TN -- Course Co-Director
Matthew MERRITT, PhDDallas, TX
Elizabeth PARKS, PhDColumbia, MO
Stephen PREVIS, PhDRahway, NJ
Michelle PUCHOWICZ, PhDMemphis, TN
Robert R. WOLFE, PhDLittle Rock, AR -- Course Co-Director
Jamey YOUNG, PhDNashville, TN

Sponsored by:
This course is supported by the Mouse Metabolic Phenotyping Centers (by grants 5U24DK076169-09 and DK115255).

Course Information
2017 Course Syllabus:
Click here to download course syllabus.

  • Registration opens on June 6, 2017
  • Registration is available online (Register click here) Registration is limited to 90 participants
  • Course registration deadline: Monday, September 30, 2017
  • Please refer to ‘Registration link’ for list of fees (credit cards accepted)
  • Registration fee includes:
    ~ breakfast, lunch and snacks
  • Please bring a Wi-Fi enable device to download and view all course material.
  • NOTE: The following book is also very useful "Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis" by Robert R. Wolfe and David L. Chinkes, 2nd Edition (2005 Wiley-Liss).
  • We have a small number of scholarships available for postdoctoral fellows or students to cover the registration fee. If you want to be considered for a scholarship, please register. Then send a no more than one page explanation of how the course would benefit your training in metabolic research as well as a letter from your mentor indicating your eligibility to by 08.14.2017 (You will be notified by 8.18.17). Please do not pay for the registration until you hear from us regarding if you have been awarded the scholarship as we have limited ability to refund credit card transactions.

To book a room at the discount rate, please call 1(615)320-7772 or go directly to the following link:
and mention the identifier:
10th Annual Tracer Course + Dates of course

Reservations must be made on or before 9/30/17