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Announcing an NIH/MMPC sponsored course:
12th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis
October 21 - October 25, 2019
A week-long course (with one-on-one mentoring sessions) in the theory and practice of stable and radioactive isotopic tracers for the study of metabolism in human and animal models.
Course Co-Directors: Owen P. McGuinness, PhD, Elizabeth J. Parks, PhD
Course Location: Homewood Suites by Hilton Nashville Vanderbilt
2400 West End Avenue
Nashville, TN 37203
615-340-8000
Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)
Welcome to the Twelfth annual course which provides basic introductory and comprehensive information on performing metabolic studies using tracers
labeled with radioactive or stable isotopes, in humans and in animals. The course is designed for beginners as well as for those with experience who wish to expand their capabilities to more sophisticated problems. The faculty has expertise in a wide variety of applications and methodologies. Techniques are taught for the investigation of whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation in humans, animals and cells. Basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR are discussed, including examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Attendees can discuss their research projects in one-on-one mentoring sessions with faculty
Monday, October 21, 2019
Basic tracer theory
Basic characteristics of radioactive isotopes
General principles of mass spectrometry
Specific activity and isotopic enrichment (GC-MS)
Methods of mass spectrometry analysis MS, LC-MS
Problem solving sessions
Tuesday, October 22, 2019
Tracer kinetics (single pool models)
Oxidation and synthesis rates
Glucose metabolism (clamp studies)
Lipid metabolism (basic kinetics)
Introduction to the NIH grant process
Practical application of the insulin clamp (breakout session)
Wednesday, October 23, 2019
Using positional isotopomer analysis to assess pathway fluxes using NMR
Protein metabolism
Thursday, October 24, 2019
Energy expenditure with doubly labeled water
Synthesis rates with deuterated water: proteins, fatty acids sterols, glucose, nucleic acids
Mass isotopomer distribution analysis: polymer synthesis, multiple flux pathways, TCA cycle, anaplerosis
Metabolic flux analysis workshop using MFA software suite
Friday, October 25, 2019
Small Group sessions
- Working with Mass Spec data
- Lipid flux
- Carbohydrate flux
- Protein flux
Pathway discovery via association of isotopomer analysis and metabolomics.
Discussion of inherently difficult problems
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@NationalMMPC
Mouse Phenotyping