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Isotope Tracers in Metabolic Research
Announcing an NIH/MMPC sponsored course: 12th Annual Course on
Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis

October 21 - October 25, 2019

A week-long course (with one-on-one mentoring sessions) in the theory and practice of stable and radioactive isotopic tracers for the study of metabolism in human and animal models.

Course Co-Directors: Owen P. McGuinness, PhD, Elizabeth J. Parks, PhD

Course Location: Homewood Suites by Hilton Nashville Vanderbilt
2400 West End Avenue
Nashville, TN 37203
615-340-8000

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

Welcome to the Twelfth annual course which provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in humans and in animals. The course is designed for beginners as well as for those with experience who wish to expand their capabilities to more sophisticated problems. The faculty has expertise in a wide variety of applications and methodologies. Techniques are taught for the investigation of whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation in humans, animals and cells. Basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR are discussed, including examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Attendees can discuss their research projects in one-on-one mentoring sessions with faculty

Monday, October 21, 2019

Basic tracer theory
Basic characteristics of radioactive isotopes
General principles of mass spectrometry
Specific activity and isotopic enrichment (GC-MS)
Methods of mass spectrometry analysis MS, LC-MS
Problem solving sessions

Tuesday, October 22, 2019

Tracer kinetics (single pool models)
Oxidation and synthesis rates
Glucose metabolism (clamp studies)
Lipid metabolism (basic kinetics)
Introduction to the NIH grant process
Practical application of the insulin clamp (breakout session)
  • Human
  • Animals

Wednesday, October 23, 2019

Using positional isotopomer analysis to assess pathway fluxes using NMR
Protein metabolism

Thursday, October 24, 2019

Energy expenditure with doubly labeled water
Synthesis rates with deuterated water: proteins, fatty acids sterols, glucose, nucleic acids
Mass isotopomer distribution analysis: polymer synthesis, multiple flux pathways, TCA cycle, anaplerosis
Metabolic flux analysis workshop using MFA software suite

Friday, October 25, 2019

Small Group sessions
  • Working with Mass Spec data
  • Lipid flux
  • Carbohydrate flux
  • Protein flux
Pathway discovery via association of isotopomer analysis and metabolomics.
Discussion of inherently difficult problems

FOR COURSE DETAILS OR SUGGESTIONS: Eann Malabanan (isotope.tracer@vanderbilt.edu) (615-343-1065)



Director & Faculty Information
Faculty:
Julio AYALA, PhDCleveland, OH
Henri BRUNENGRABER, MD PhDCleveland, OH
Stephanie CHUNG, MBBSBethesda, MD
Melanie CREE GREEN, MD PhDDenver, CO
Joanne KELLEHER, PhDBoston, MA
Maren LAUGHLIN, PhDBethesda, MD
Owen P. McGUINNESS, PhDNashville, TN -- Course Co-Director
Matthew MERRITT, PhDDallas, TX
Elizabeth J. PARKS, PhDColumbia, MO -- Course Co-Director
Stephen PREVIS, PhDRahway, NJ
Michelle PUCHOWICZ, PhDMemphis, TN
David H. WASSERMAN, PhDNashville, TN
Robert R. WOLFE, PhDLittle Rock, AR
Jamey YOUNG, PhDNashville, TN

Sponsored by:
This course is supported by The National Institute of Diabetes and Digestive and Kidney Diseases and Mouse Metabolic Phenotyping Centers.

Course Information
2019 Course Syllabus: Click here to download course syllabus.

COURSE REGISTRATION & MATERIAL
  • Registration link (opens June 1, 2019): https://is.gd/TracerCourse2019
  • Course registration deadline: Monday, October 1, 2019
  • Please refer to 'Registration link' for list of fees (credit cards accepted)
  • Registration fee includes lunch, snacks, and one welcome dinner
  • Please bring a Wi-Fi enable device to download and view all course material
  • NOTE: The following book is also very useful "Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis" by Robert R. Wolfe and David L. Chinkes, 2nd Edition (2005 Wiley-Liss).
  • We have a small number of scholarships available for postdoctoral fellows or students to cover the registration fee. If you want to be considered for a scholarship, please register and follow the instructions. Send no more than a one-page explanation of how the course would benefit your training in metabolic research as well as a letter from your mentor indicating your eligibility to isotope.tracer@vanderbilt.edu by August 15, 2019. You will be notified by September 1, 2019, if you are a scholarship recipient. Please do not pay for the registration until you hear from us as to whether you have been awarded the scholarship. We have limited ability to refund credit card transactions.

ACCOMMODATIONS & INFORMATION
Hotel Reservations: A block of rooms has been reserved at the course location (Homewood Suites, Nashville-Vanderbilt) at a rate of $189 per night. Please book by Oct. 4th to secure this rate.
You can reserve by calling 1-615-340-8000 and referencing Group Code "VT9" or by following the direct booking link:
https://homewoodsuites.hilton.com/en/hw/groups/personalized/B/BNAVBHW-VT9-20191020/index.jhtml?WT.mc_id=POG

Check-in/out: 20-OCT-2019 / 25-OCT-2019

Hotel Address:
2400 West End Avenue
Nashville, Tennessee 37203

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