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Isotope Tracers in Metabolic Research

Isotope Tracers in Metabolic Research: A 3-Part Webinar Series.


Organized by the University of Missouri, Vanderbilt University and the NIDDK.
September 15th, October 20th and November 17th, 2020.
3 Part Webinar Clear here for more information.
Please note each part requires a separate registration (see flyer for details).


Part 1: TUES, SEPT. 15 | 2 - 3 PM CST
Use of lipid tracers to track fatty acid flux in humans and animal models of NAFLD
Elizabeth Parks, PhD (University of Missouri School of Medicine)
*Register for Part 1: https://redcap.vanderbilt.edu/surveys/?s=C8L73KCN4M
Link to seminar video: https://youtu.be/iQV1WcrmtUQ

Part 2: TUES, OCT. 20 | 2 - 3 PM CST
Tracing hepatic glucose and glycogen metabolism in metabolic disease
Owen McGuinness, PhD (Vanderbilt University School of Medicine)
*Register for Part 2: https://redcap.vanderbilt.edu/surveys/?s=M8N4JKT4MA
Link to seminar video: https://www.youtube.com/watch?v=_-hwfSwykP4


https://www.mmpc.org/shared/courses.aspx#webinar

Part 3: TUES, NOV. 17 | 2 - 3 PM CST
Central metabolism is a target of Pioglitazone action
Matt Merritt, PhD (University of Florida College of Medicine)
*Register for Part 3: https://redcap.vanderbilt.edu/surveys/?s=L7EL3NKLYX

*Connection information differs per webinar and will be provided after registration

Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis

Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis

13th Annual Course
April 12 - 16, 2021

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

Course Co-Directors: Owen P. McGuinness, PhD, Elizabeth J. Parks, PhD

Location: Homewood Suites by Hilton Nashville Vanderbilt
2400 West End Avenue
Nashville, TN 37203
615-340-8000

Overview: A week-long course (with one-on-one mentoring sessions) in the theory and practice of stable and radioactive isotopic tracers for the study of metabolism in human and animal models.


FOR COURSE DETAILS OR SUGGESTIONS: Eann Malabanan at isotope.tracer@vanderbilt.edu or 615-343-1065

Course Information
This course provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes in humans, animals and cells. The course is designed for beginners as well as for those with experience who wish to expand their capabilities to more sophisticated problems. The faculty has expertise in a wide variety of applications and methodologies.

Techniques are taught for the investigation of whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation in humans, animals and cells. Basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR are discussed, including examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Attendees will have opportunities to discuss their research projects in one-on-one mentoring sessions with faculty.



Course Syllabus Example: Download 2019 Course Syllabus

Course Overview (tentative)

Monday, April 12, 2021

Basic tracer theory
Basic characteristics of radioactive isotopes
General principles of mass spectrometry
Specific activity and isotopic enrichment (GC-MS)
Methods of mass spectrometry analysis MS, LC-MS
Problem solving sessions

Tuesday, April 13, 2021

Tracer kinetics (single pool models)
Oxidation and synthesis rates
Glucose metabolism (clamp studies)
Lipid metabolism (basic kinetics)
Introduction to the NIH grant process
Practical application of the insulin clamp (breakout session)
  • Human
  • Animals

Wednesday, April 14, 2021

Using positional isotopomer analysis to assess pathway fluxes using NMR
Protein metabolism

Thursday, April 15, 2021

Energy expenditure with doubly labeled water
Synthesis rates with deuterated water: proteins, fatty acids sterols, glucose, nucleic acids
Mass isotopomer distribution analysis: polymer synthesis, multiple flux pathways, TCA cycle, anaplerosis
Metabolic flux analysis workshop using MFA software suite

Friday, April 16, 2021

Small Group sessions
  • Working with Mass Spec data
  • Lipid flux
  • Carbohydrate flux
  • Protein flux
Pathway discovery via association of isotopomer analysis and metabolomics.
Discussion of inherently difficult problems

Registration & Accommodations
REGISTRATION
  • Registration form: TBA
    • Refer to registration link for registration fees and payment instructions. Registration fee includes lunch, snacks, and one welcome dinner.
  • Registration opens: TBA
  • Registration deadline: TBA
Scholarships for graduate students and postdoctoral fellows to cover registration fees are available.
If you would like to be considered, follow the instructions on the registration link. Send no more than a one-page explanation of how the course would benefit your training in metabolic research as well as a letter from your mentor indicating your eligibility to isotope.tracer@vanderbilt.edu by TBD. Scholarship recipients will be notified by TBD. Please do not pay for the registration until you hear from us as to whether you have been awarded the scholarship. We have limited ability to refund credit card transactions.
MATERIALS
  • Bring a Wi-Fi enabled device to download and view all course material
  • The following book is useful but not required: Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis by Robert R. Wolfe and David L. Chinkes, 2nd Edition (2005 Wiley-Liss)
ACCOMODATIONS
  • Hotel Reservations: A block of rooms has been reserved at the course location (Homewood Suites, Nashville-Vanderbilt) at a rate of $199 per night. Please book by March 11, 2021 to secure this rate.
  • You can reserve by calling 1-615-340-8000 and referencing Group Code "VUT" or by following the direct booking link: TBA
Check-in/out: 11-APRIL-2021 / 15-APRIL-2021
Hotel Address (location of the course):
2400 West End Avenue
Nashville, Tennessee 37203

Director & Faculty Information
Faculty:
Julio AYALA, PhDCleveland, OH
Henri BRUNENGRABER, MD PhDCleveland, OH
Stephanie CHUNG, MBBSBethesda, MD
Melanie CREE GREEN, MD PhDDenver, CO
Timothy GARRETT, PhDGainesville, FL
Joanne KELLEHER, PhDBoston, MA
Maren LAUGHLIN, PhDBethesda, MD
Owen P. McGUINNESS, PhDNashville, TN -- Course Co-Director
Matthew MERRITT, PhDDallas, TX
Elizabeth J. PARKS, PhDColumbia, MO -- Course Co-Director
Stephen PREVIS, PhDRahway, NJ
Michelle PUCHOWICZ, PhDMemphis, TN
David H. WASSERMAN, PhDNashville, TN
Robert R. WOLFE, PhDLittle Rock, AR
Jamey YOUNG, PhDNashville, TN

Sponsored by:
The National Institute of Diabetes and Digestive and Kidney Diseases and Mouse Metabolic Phenotyping Centers

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