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Catalog Item
Lipid extraction from liver
Common Name(s): Lipid Analysis

CenterUniversity of California Davis
Center CoreM&MH Core
Test No.D3101
Test Priceinquire
DescriptionTriglyceride and cholesterol content in liver tissue will be determined by the
Folch method (Folch, 1957). Weighed tissue samples are homogenized in
methanol:chloroform. After overnight extraction, 0.7% sodium chloride is added.
The aqueous layer is aspirated and duplicate aliquots of the chloroform/lipid
layer are dried under nitrogen gas. The lipid is reconstituted in isopropyl
alcohol and assayed for TG spectrophotometrically with enzymatic reagents from
Fisher Diagnostics (Middletown, VA)
DataData will be collected for this catalog item
Keywordsatherosclerosis, cholesterol, obesity, total cholesterol, triglycerides
Tissuesliver, liver epithelium



Catalog Groups



Phenotype Assays
NameAbbreviationOptions
cholesterol, tissue
cholesterol (tissue)
View
triglycerides, tissue
TG (tissue)
View




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