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Catalog Item
ICV cannulation

CenterVanderbilt University School of Medicine
Center CoreMetabolic Regulation Core
Test No.V3001
Test Price$61
DescriptionImplantation of a cerebral ventricle cannula allows investigators to evaluate
physiological responses following central administration of various compounds.
Anesthetized mice are placed in a digital stereotaxic apparatus (0.001 mm
accuracy, Cartesian Instruments) specifically designed for mice. The dorsal
scalp will be shaved, wiped with a betadine solution, and then a small midline
incision over the dorsal surface is made to allow access to the cranium. After
the affixed centering scope is used to "zero" lambda and bregma landmarks, a
single guide cannula (2.5 mm length, 26-gauge, Plastics One) is positioned 1.0
mm above the lateral ventricle (coordinates: 0.6 mm posterior to bregma, 1.5 mm
lateral to midline, 1.4 mm below the surface of the skull) and fixed to the
skull using two stainless steel screws and dental cement. The incision in the
scalp is then closed with surgical thread. Animals are removed to a
post-surgical warming bed, and then individually housed for several hours until
fully awake. Animals will be allowed to recover from surgery for a minimum of 7
days prior to testing, during which time a 30-gauge dummy cannula is left inside
the guide cannula to prevent blockage.
DataNo data will be collected for this catalog item
Keywordscentral control, CSF, surgery
Tissuesbrain



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