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Catalog Item
Amino acid kinetics
Common Name(s): Amino Acids

CenterVanderbilt University School of Medicine
Center CoreMetabolic Regulation Core
Test No.V3009
Test PriceConsult with Director
DescriptionThe turnover of phenyalanine (3H ring 2,3,4,5,6 phenylalanine) , glutamine
(U-14C-glutamine) and leucine (1-14C-leucine) is assessed by a primed continuous
infusion of their respective isotopes for 2 hours (0.2-0.4 µCi/min). Blood
samples (20 µl) are taken after a steady state is reached to assess plasma amino
acid specific activity. Blood samples are mixed with an equal volume of 6%
sulfosalicylic acid. Incorporation of tracer in tissue protein is used to assess
tissue specific protein synthesis.
DataData will be collected for this catalog item
Keywordsamino acid flux, amino acid kinetics, isotopes, metabolite
Tissues



Catalog Groups



Phenotype Assays


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