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Catalog Item
Tissue specific glucose uptake
Common Name(s): 2DG uptake

CenterVanderbilt University School of Medicine
Center CoreMetabolic Regulation Core
Test No.V3010
Test PriceConsult with Director
DescriptionTissue specific glucose uptake is assessed by measuring the tissue specific
uptake of [2- 3H]-deoxyglucose([2-3H]DG). [2-3H]DG is infused (0.2 µCi/min) for
40 minutes or injected (12 µCi) . Arterial plasma samples are taken to determine
the time course of [2-3H]DG during the 40 min period. [2-3H]DG is transported
into cells and phosphorylated to yield [2-3H]DG-6-phosphate which is trapped in
muscle. After 40 min mice are anesthetized with an intravenous infusion of
pentobarbital and tissues of interest are rapidly removed and frozen in liquid
nitrogen. This method has been applied during insulin- and exercise-stimulated
conditions (Halseth et al. Am. J. Physiol. 1999).
DataData will be collected for this catalog item
Keywords2-deoxyglucose, carbohydrate, diabetes, glucose metabolic index, insulin,
insulin action, insulin sensitivity, tissue specific glucose uptake
Tissuesadipose tissue, brain, heart, skeletal muscle



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