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Experiment

Circulating insulin, lipid, ALT, and AST levels in B6 mice fed high fat diet and
treated with niclosamide
Summary Data Summary
Investigator Shengkan, Jin
Description "Oral niclosamide ethanolamine (NEN) increases energy expenditure and lipid
metabolism in mice. It is also efficacious in preventing and treating hepatic
steatosis and insulin resistance induced by a high-fat diet. Moreover, it
improves glycemic control and delays disease progression in db/db mice. Given
the well-documented safety profile of NEN, it provides a potentially new and
practical pharmacological approach for treating T2D.

5 week old male C57BL/6J mice were treated with high fat diet(HFD) for 21 weeks.
MB1-47 and MB1-78 both derivatives of niclosamide ethanolamine were added to the
diet at concentrations of 150mg and 300mg per kg of diet. After 5 hour fasting,
mice were anesthetized with ketamine and xylazine to draw blood from heart with
heparin-coated tube. Plasma was obtained by centrifugation at 1000g for 10-15min
at 4? , and stored at -80? for further analysis."

Applicable research area(s): Diabetes, Metabolism, Endocrine deficiency
Status Completed
Public Release 8/8/2019
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainNo Experimental GroupNeed Conditions
Data Analysis
TypeCount
Animals21
Experimental Conditions5
Catalog Items6
Curation Info (# flags)3
Phenotype Assays6
Phenotype Measurements126
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
21
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat
 Research Diets - D16121601
Rodent Diet with 60% kcal fat, 20% kcal carbohydrate, 20% kcal protein supplemented with 300 mg/kg MB1-47, a deriviative of niclosamide ethanolamine (NEN)
 Research Diets - D17041902
Rodent Diet with 60% kcal fat, 20% kcal carbohydrate, 20% kcal protein supplemented with 150 mg/kg MB1-78, a deriviative of niclosamide ethanolamine (NEN)

Experimental Factor: Tissue Source

 plasma (sodium heparin)
plasma (sodium heparin)



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