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Analytical Study in Ntambi Mice 12034
The skin is the single largest organ in humans, serving as a major barrier to
infection, water loss, and abrasion. The functional diversity of skin requires
the synthesis of large amounts of lipids, such as triglycerides, wax esters,
squalene, ceramides, free cholesterol, free fatty acids, and cholesterol and
retinyl esters. Some of these lipids are used as cell membrane components,
signaling molecules, and a source of energy. An important class of lipid
metabolism enzymes expressed in skin is the ?(9)-desaturases, which catalyze the
synthesis in ?(9)-monounsaturated lipids, primarily oleoyl-CoA (18:1n-9) and
palmitoyl-CoA (16:1n-7), the major monounsaturated fatty acids in cutaneous
Mice with a deletion of the ?(9)-desaturase-1 isoform (SCD1) either globally
(Scd1(-/-)) or specifically in the skin (skin-specific Scd1-knockout; SKO)
present with marked changes in cutaneous lipids and skin integrity.
Interestingly, these mice also exhibit increased whole body energy expenditure,
protection against diet-induced adiposity, hepatic steatosis, and glucose
intolerance. The increased energy expenditure in skin-specific Scd1-knockout
(SKO) mice is a surprising phenotype, as it links cutaneous lipid homeostasis
with whole body energy balance.
Liver-specific knockout of Scd1(LKO) mice were protected from high-carbohydrate,
but not high-fat (HF), diet-induced adiposity and hepatic steatosis.
Additionally, on a high-sucrose, very low-fat (HSVLF) diet, lipogenesis and
levels of nuclear SREBP-1 and ChREBP were significantly decreased in the livers
of LKO relative to Scd1(lox/lox) (Lox) mice. HSVLF feeding in LKO mice caused
hypoglycemia and hepatic carbohydrate reduction due to an impairment of
gluconeogenesis. Oleate, but not stearate, supplementation normalized adiposity,
gluconeogenesis, triglyceride secretion, and hepatic lipogenesis of LKO mice.
Applicable research area(s): Diabetes, Metabolism, Obesity, Endocrine deficiency
week(s) post-natal (w)
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Curation Info (# flags)
LKO (liver-specific ko)
Scd1 global KO
Name / Abbreviation
Experimental Factor: Experimental Group
This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.
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macrophage colony stimulating factor, granulocyte
Interleukin 1 alpha
Interleukin 1 beta
Interleukin 8 homologue
Tumor necrosis factor alpha
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Mammalian Phenotype (MP) Terms
decreased circulating leptin level
less than the normal blood concentration of the peptide hormone secreted by white adipocytes and believed to regulate food intake and energy balance [MESH:D06.472.699.042.500]
MMPC Search Results
increased circulating leptin level
greater than the normal blood concentration of the peptide hormone secreted by white adipocytes and believed to regulate food intake and energy balance [MESH:D06.472.699.042.500]
MMPC Search Results
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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