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Experiment

Analytical Study in Ntambi Mice 12034
Summary Data Summary
Investigator Ntambi, James
Description The skin is the single largest organ in humans, serving as a major barrier to
infection, water loss, and abrasion. The functional diversity of skin requires
the synthesis of large amounts of lipids, such as triglycerides, wax esters,
squalene, ceramides, free cholesterol, free fatty acids, and cholesterol and
retinyl esters. Some of these lipids are used as cell membrane components,
signaling molecules, and a source of energy. An important class of lipid
metabolism enzymes expressed in skin is the ?(9)-desaturases, which catalyze the
synthesis in ?(9)-monounsaturated lipids, primarily oleoyl-CoA (18:1n-9) and
palmitoyl-CoA (16:1n-7), the major monounsaturated fatty acids in cutaneous
lipids.

Mice with a deletion of the ?(9)-desaturase-1 isoform (SCD1) either globally
(Scd1(-/-)) or specifically in the skin (skin-specific Scd1-knockout; SKO)
present with marked changes in cutaneous lipids and skin integrity.
Interestingly, these mice also exhibit increased whole body energy expenditure,
protection against diet-induced adiposity, hepatic steatosis, and glucose
intolerance. The increased energy expenditure in skin-specific Scd1-knockout
(SKO) mice is a surprising phenotype, as it links cutaneous lipid homeostasis
with whole body energy balance.

Liver-specific knockout of Scd1(LKO) mice were protected from high-carbohydrate,
but not high-fat (HF), diet-induced adiposity and hepatic steatosis.
Additionally, on a high-sucrose, very low-fat (HSVLF) diet, lipogenesis and
levels of nuclear SREBP-1 and ChREBP were significantly decreased in the livers
of LKO relative to Scd1(lox/lox) (Lox) mice. HSVLF feeding in LKO mice caused
hypoglycemia and hepatic carbohydrate reduction due to an impairment of
gluconeogenesis. Oleate, but not stearate, supplementation normalized adiposity,
gluconeogenesis, triglyceride secretion, and hepatic lipogenesis of LKO mice.

Applicable research area(s): Diabetes, Metabolism, Obesity, Endocrine deficiency
Status Completed
Public Release 9/25/2016
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagExperiment / Control
Data Analysis
TypeCount
Animals26
Experimental Conditions1
Catalog Items1
Curation Info (# flags)1
Phenotype Assays11
Phenotype Measurements286
Histology Images0
Publications0
Phenotypes2


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
4
0
Scd1 SKO
0
8
0
LKO (liver-specific ko)
0
3
0
Scd1 global KO
0
11
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.



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