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Experiment

Energy Balance and Max Test #18459 9/29/14
Summary Data Summary
Investigator Wasserman, David
Description During the first week mice received energy expenditure measured by indirect
calorimetry in the Promethion by Sable Systems. Activity, food intake and water
intake are also continuously measured.
Measurements were taken for 5 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.

Groups:
Male WT (C56BL/6J +/+ ) and KO (C57BL/6J-Sirt3tm1Was) mice.
Mice were fed a chow diet (LabDiets 5001).

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and feeding behaviors.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.

During the second week mice received energy expenditure measured by indirect
calorimetry in the Oxymax by Columbus Instruments. Mice were trained to run on
the treadmill by running them on a treadmill for 10 minutes at 10 m/min 2 to 3
days prior to the experiment. On the day of the experiment mice were placed in
the treadmill attached to the oxymax. The treadmill was turned off for 30
minutes to take readings of the animal rested. Mice were then run on a treadmill
at gradually increasing speeds until exhaustion. When the mice became exhausted
the treadmill was turned off and recordings were taken for another 30 minutes.
Speeds were increased by 4 m/min every 3 minutes, with the starting speed set to
10 m/min.

Groups:
Male WT (C56BL/6J +/+ ) and KO (C57BL/6J-Sirt3tm1Was) mice.
Mice were fed a chow diet (LabDiets 5001).

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and different exercise capacity.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure.
Status Completed
Public Release 3/12/2017
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions3
Catalog Items2
Curation Info (# flags)1
Phenotype Assays15
Phenotype Measurements368
Histology Images0
Publications0
Phenotypes2


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
0
7
0
C57BL/6J-Sirt3-/-
0
9
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Sable Run (RNum)

 Sable Run
The run number for the Sable system used to assess energy expenditure.

Experimental Factor: Treadmill Speed (m/min)

 Treadmill_Speed
The speed of the treadmill used during an energy expenditure experiment



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